Product Code: PIC2008_P244

Micro Cutting of Madin-darby Canine Kidney (MDCK) Cell using Femtosecond Laser
Authors:
Jianfeng Liu, University of Science and Technology of China; Hefei Anhui Peoples Republic of China
Rui Guo, University of Science and Technology of China; Hefei Anhui Peoples Republic of China
Xiang Wang, University of Science and Technology of China; Hefei Anhui Peoples Republic of China
Zhen Guo, University of Science and Technology of China; Hefei Anhui Peoples Republic of China
Wenhao Huang, University of Science and Technology of China; Hefei Anhui China Peoples Republic of China
Presented at PICALO 2008

Femtosecond Laser is a unique tool for micro and nano fabrication. In this paper, we will discuss its application in micro cutting of live cells. It includes the interaction of femtosecond laser and Madin-Darby canine kidney (MDCK) cell, the optimization of cutting parameters such as laser energy and exposure time, the process of the micro cutting of MDCK cell's pseudopod. Furthermore, the cell's living activity of shrinking after cutting is observted and analyzed.The result demonstrates the feasibility of nanosurgery in live cells with femtosecond laser microfabrication technology by selectivity cutting without compromising the activity of the cells.