Authors:
V. K. Unnikrishnan
Rajesh Nayak
Rodney Bernard
Centre for Atomic and Molecular Physics, Manipal University, Manipal, 576 104, India

K. Jeena Priya
Department of Oral Medicine and Radiology, MCODS Mangalore, Manipal University, Manipal, 575 001, India

Ajeetkumar Patil
Centre for Atomic and Molecular Physics, Manipal University, Manipal, 576 104 India

J. Ebenezer
PG & Research Department of Physics, Jamal Mohamed College, Tiruchinapalli, Manipal, 620 020, India

Keerthilatha M. Pai
Department of Oral Medicine and Radiology, MCODS Manipal, Manipal University, Manipal,576 104 India

Sajan D. George
Center of Smart Interfaces, TU Darmstadt, Petersensstrasse 32, Darmstadt, 64287, Germany

V. B. Kartha
21 Udayagiri, Sion, Trombay Road, Mumbai, 400 088, India

C. Santhosh
Centre for Atomic and Molecular Physics, Manipal University, Manipal, 576 104 India

Despite the advances in optical technologies for early detection of cancer, routine clinical applications are still not standardized. Among several optical methods, laser-induced fluorescence is a more matured and well understood technique. Still due care has to be taken about various factors to avoid erroneous results. The authors have carried out a systematic study on the effect of the various experimental parameters such as source stability, area of exposure, and angular/distance dependence of a fiber probe, which is used for the fluorescence measurements, from the specimen surface on the spectra. Investigations are carried out on both idealistic cases as well as on tissue surface. The details of the optimization of the parameters are presented and discussed in this paper.