Authors:
K. Wallat
Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaetsstr. 5-7, 45117 Essen, Germany

D. Do¨rr
R. Le Harzic
F. Stracke
D. Sauer
Fraunhofer Institute for Biomedical Engineering (IBMT) and Chair for Molecular and Cellular Biotechnology (University of Saarbruecken), Ensheimer Str. 48, 66386 St. Ingbert, Germany

M. Neumeier
A. Kovtun
Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaetsstr. 5-7, 45117 Essen, Germany

H. Zimmermann
Fraunhofer Institute for Biomedical Engineering (IBMT) and Chair for Molecular and Cellular Biotechnology (University of Saarbruecken), Ensheimer Str. 48, 66386 St. Ingbert, Germany

M. Epple
Institute of Inorganic Chemistry and Center for Nanointegration Duisburg-Essen (CeNIDE), University of Duisburg-Essen, Universitaetsstr. 5-7, 45117 Essen, Germany

Silicon wafers were structured with a femtosecond laser on the cm² scale with high spatial frequency laser-induced periodic surface structures. These areas are characterized by regular parallel ripples with a period of the order of 100 nm. The particular ripple spacing is determined by the illumination wavelength of the tunable femtosecond laser. The cellular reaction to the structured silicon wafers and to the same materials, coated with calcium phosphate nanoparticles by electrophoretic deposition, was studied using L929 fibroblasts, human mesenchymal stem cells, and epithelial cells. The cells adhered uniformly to structured and unprocessed areas after seeding but significantly preferred the unstructured silicon after 48 h. This behavior disappeared after coating the structured surface with calcium phosphate nanoparticles.